Use of rpsL for dominance selection and gene replacement in Streptomyces roseosporus.
We developed a gene replacement system using the rpsL gene of Streptomyces roseosporus and demonstrated its utility by constructing a deletion in the S. roseosporus glnA gene. A 1.3-kb BamHI fragment that hybridized to the Mycobacterium smegmatis rpsL gene was subcloned from an S. roseosporus cosmid...
Gespeichert in:
| Veröffentlicht in: | J Bacteriol |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology (ASM)
1997
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC178677/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8981996/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.179.1.180-186.1997 |
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