The processive endocellulase CelF, a major component of the Clostridium cellulolyticum cellulosome: purification and characterization of the recombinant form.

The recombinant form of the cellulase CelF of Clostridium cellulolyticum, tagged by a C-terminal histine tail, was overproduced in Escherichia coli. The fusion protein was purified by affinity chromatography on a Ni-nitrilotriacetic acid column. The intact form of CelF (Mr, 79,000) was rapidly degra...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Reverbel-Leroy, C, Pages, S, Belaich, A, Belaich, J P, Tardif, C
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1997
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC178660/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8981979/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.179.1.46-52.1997
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