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Mutational analysis of nucleoside diphosphate kinase from Pseudomonas aeruginosa: characterization of critical amino acid residues involved in exopolysaccharide alginate synthesis.

We report the utilization of site-directed and random mutagenesis procedures in the gene encoding nucleoside diphosphate kinase (ndk) from Pseudomonas aeruginosa in order to examine the role of Ndk in the production of alginate by this organism. Cellular levels of the 16-kDa form of the Ndk enzyme a...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Sundin, G W, Shankar, S, Chakrabarty, A M
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1996
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC178623/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8955392/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.178.24.7120-7128.1996
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