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Site-specific DNA transesterification catalyzed by a restriction enzyme
Most restriction endonucleases use Mg(2+) to hydrolyze phosphodiester bonds at specific DNA sites. We show here that BfiI, a metal-independent restriction enzyme from the phospholipase D superfamily, catalyzes both DNA hydrolysis and transesterification reactions at its recognition site. In the pres...
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| Autores principales: | , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
2007
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1785359/ https://ncbi.nlm.nih.gov/pubmed/17267608 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0608689104 |
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