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Cloning, sequencing, and overexpression of the genes encoding coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii.

The genes encoding coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii were cloned and overexpressed in Escherichia coli. The B12-free enzyme was purified to homogeneity. It consists of three types of subunits whose N-terminal sequences are in accordance with those deduced from the o...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Bacteriol
Hauptverfasser: Seyfried, M, Daniel, R, Gottschalk, G
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1996
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC178423/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8824629/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.178.19.5793-5796.1996
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