Isolation and characterization of rheumatoid arthritis synovial fibroblasts from primary culture — primary culture cells markedly differ from fourth-passage cells

To reduce culture artifacts by conventional repeated passaging and long-term culture in vitro, the isolation of synovial fibroblasts (SFB) was attempted from rheumatoid arthritis (RA) synovial membranes by trypsin/collagenase digest, short-term in vitro adherence (7 days), and negative isolation usi...

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Podrobná bibliografie

Vydáno v:	Arthritis Res
Hlavní autoři:	Zimmermann, Thomas, Kunisch, Elke, Pfeiffer, Robert, Hirth, Astrid, Stahl, Hans-Detlev, Sack, Ulrich, Laube, Anke, Liesaus, Eckehard, Roth, Andreas, Palombo-Kinne, Ernesta, Emmrich, Frank, Kinne, Raimund W
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	BMC 2000
Témata:	Primary Research
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC17827/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11178129/ https://ncbi.nlm.nih.govhttps://doi.org/10.1186/ar142
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