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Isolation and characterization of rheumatoid arthritis synovial fibroblasts from primary culture — primary culture cells markedly differ from fourth-passage cells

To reduce culture artifacts by conventional repeated passaging and long-term culture in vitro, the isolation of synovial fibroblasts (SFB) was attempted from rheumatoid arthritis (RA) synovial membranes by trypsin/collagenase digest, short-term in vitro adherence (7 days), and negative isolation usi...

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Udgivet i:Arthritis Res
Principais autores: Zimmermann, Thomas, Kunisch, Elke, Pfeiffer, Robert, Hirth, Astrid, Stahl, Hans-Detlev, Sack, Ulrich, Laube, Anke, Liesaus, Eckehard, Roth, Andreas, Palombo-Kinne, Ernesta, Emmrich, Frank, Kinne, Raimund W
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2000
Fag:
Primary Research
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC17827/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11178129/
https://ncbi.nlm.nih.govhttps://doi.org/10.1186/ar142
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