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Membrane topology of the outer membrane protein OprH from Pseudomonas aeruginosa: PCR-mediated site-directed insertion and deletion mutagenesis.

The 21-kDa outer membrane protein OprH from Pseudomonas aeruginosa is overexpressed under Mg2+ starvation conditions and when overproduced causes resistance to polymyxin B, gentamicin, and EDTA. By circular dichroism analysis, OprH revealed a calculated beta-sheet structure content of 47.3%. PCR-bas...

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Bibliografiske detaljer
Main Authors: Rehm, B H, Hancock, R E
Format: Artigo
Sprog:Inglês
Udgivet: 1996
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC178091/
https://ncbi.nlm.nih.gov/pubmed/8655519
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