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Characterization of heterologously produced carbonic anhydrase from Methanosarcina thermophila.

The gene encoding carbonic anhydrase from Methanosarcina thermophila was hyperexpressed in Escherichia coli, and the heterologously produced enzyme was purified 14-fold to apparent homogeneity. The enzyme purified from E. coli has properties (specific activity, inhibitor sensitivity, and thermostabi...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Alber, B E, Ferry, J G
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1996
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC178080/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8655508/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.178.11.3270-3274.1996
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