Site-specific proteolysis of the Escherichia coli SecA protein in vivo.
A seven-amino-acid cleavage site specific for tobacco etch virus (TEV) protease was introduced into SecA at two separate positions after amino acids 195 and 252. Chromosomal wild-type secA was replaced by these secA constructs. Simultaneous expression of TEV protease led to cleavage of both SecA der...
Gardado en:
| Publicado en: | J Bacteriol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1996
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC178040/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8631693/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.178.10.2986-2988.1996 |
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