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Generation of influenza A viruses entirely from cloned cDNAs

We describe a new reverse-genetics system that allows one to efficiently generate influenza A viruses entirely from cloned cDNAs. Human embryonic kidney cells (293T) were transfected with eight plasmids, each encoding a viral RNA of the A/WSN/33 (H1N1) or A/PR/8/34 (H1N1) virus, flanked by the human...

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Published in:Proc Natl Acad Sci U S A
Main Authors: Neumann, Gabriele, Watanabe, Tokiko, Ito, Hiroshi, Watanabe, Shinji, Goto, Hideo, Gao, Peng, Hughes, Mark, Perez, Daniel R., Donis, Ruben, Hoffmann, Erich, Hobom, Gerd, Kawaoka, Yoshihiro
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1999
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC17785/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10430945/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.16.9345
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