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Isolation and characterization of a mutation that alters the substrate specificity of the Escherichia coli glucose permease.

We isolated 10 mannitol-positive mutants from a mannitol-negative Escherichia coli strain. These mutations mapped within ptsG, encoding the glucose permease (EIIGlc), and resulted in a G-320-to-V substitution that allows EIIGlc to transport mannitol. Gly-320 lies within a putative transmembrane heli...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Bacteriol
Hauptverfasser: Begley, G S, Warner, K A, Arents, J C, Postma, P W, Jacobson, G R
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1996
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177751/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8550539/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.178.3.940-942.1996
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