Overexpression, purification, and characterization of UDP-N-acetylmuramyl:L-alanine ligase from Escherichia coli.

UDP-N-acetylmuramyl:L-alanine ligase from Escherichia coli was overexpressed more than 600-fold and purified to near homogeneity. The purified enzyme was found to ligate L-alanine, L-serine, and glycine, as well as the nonnatural amino acid beta-chloro-L-alanine, to UDP-N-acetylmuramic acid. On the...

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Vydáno v:	J Bacteriol
Hlavní autoři:	Gubler, M, Appoldt, Y, Keck, W
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 1996
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177743/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8550531/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.178.3.906-910.1996
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