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Role of the ferric uptake regulator of Pseudomonas aeruginosa in the regulation of siderophores and exotoxin A expression: purification and activity on iron-regulated promoters.

The cloned Pseudomonas aeruginosa fur (ferric uptake regulator) gene was overexpressed in P. aeruginosa by using a T7 expression system, and the Fur protein (PA-Fur) was purified by using a combination of ion-exchange chromatography and metal affinity chromatography. The DNA binding activity of the...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Ochsner, U A, Vasil, A I, Vasil, M L
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1995
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177600/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8522528/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.24.7194-7201.1995
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