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Altered (copy-up) forms of initiator protein pi suppress the point mutations inactivating the gamma origin of plasmid R6K.

The R6K gamma origin core contains the P2 promoter, whose -10 and -35 hexamers overlap two of the seven binding sites for the R6K-encoded pi protein. Two mutations, P2-201 and P2-203, which lie within the -35 region of P2, are shown to confer a promoter-down phenotype. We demonstrate here that these...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Urh, M, Flashner, Y, Shafferman, A, Filutowicz, M
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1995
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC177536/
https://ncbi.nlm.nih.gov/pubmed/7592461
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