Molecular cloning with a pMEA300-derived shuttle vector and characterization of the Amycolatopsis methanolica prephenate dehydratase gene.

An efficient restriction barrier for methylated DNA in the actinomycete Amycolatopsis methanolica could be avoided by using a nonmethylating Escherichia coli strain for DNA isolations. The A. methanolica prephenate dehydratase gene was cloned from a gene bank in a pMEA300-derived shuttle vector in E...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Vrijbloed, J W, van Hylckama Vlieg, J, van der Put, N M, Hessels, G I, Dijkhuizen, L
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177523/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7592448/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.22.6666-6669.1995
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