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PCR mutagenesis identifies a polymerase-binding sequence of sigma 54 that includes a sigma 70 homology region.

Sigma 54 is a minor bacterial sigma factor that is not a member of the sigma 70 family of proteins but binds the same core RNA polymerase. Previously, we identified a region of sigma 54 that is important for binding core polymerase. In this work, PCR mutagenesis was used to identify specific amino a...

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Udgivet i:J Bacteriol
Principais autores: Tintut, Y, Gralla, J D
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1995
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177404/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7592329/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.20.5818-5825.1995
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