Cloning of the two pyruvate kinase isoenzyme structural genes from Escherichia coli: the relative roles of these enzymes in pyruvate biosynthesis.

We report the cloning of the pykA and pykF genes from Escherichia coli, which code for the two pyruvate kinase isoenzymes (ATP:pyruvate 2-O-phosphotransferases; EC 2.7.1.40) in this microorganism. These genes were insertionally inactivated with antibiotic resistance markers and utilized to interrupt...

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Vydáno v:	J Bacteriol
Hlavní autoři:	Ponce, E, Flores, N, Martinez, A, Valle, F, Bolívar, F
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 1995
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177388/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7559366/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.19.5719-5722.1995
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