Use of Tn5tac1 to clone a pel gene encoding a highly alkaline, asparagine-rich pectate lyase isozyme from an Erwinia chrysanthemi EC16 mutant with deletions affecting the major pectate lyase isozymes.

Erwinia chrysanthemi mutant CUCPB5047, delta(pelA pelE) delta(pelB pelC)::28bp delta(pelX) delta 4bp pehX::omega Cmr, was constructed, mutated with Tn5tac1, and screened for isopropyl-beta-D-thiogalactopyranoside-dependent pectate lyase (Pel) production. A Kmr SacI fragment from the hyperexpressing...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Alfano, J R, Ham, J H, Collmer, A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177214/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7635842/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.15.4553-4556.1995
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