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Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.

We have constructed a series of plasmid vectors (pBAD vectors) containing the PBAD promoter of the araBAD (arabinose) operon and the gene encoding the positive and negative regulator of this promoter, araC. Using the phoA gene and phoA fusions to monitor expression in these vectors, we show that the...

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Détails bibliographiques
Publié dans:J Bacteriol
Auteurs principaux: Guzman, L M, Belin, D, Carson, M J, Beckwith, J
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1995
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177145/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7608087/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.14.4121-4130.1995
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