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UmuD′(2)C is an error-prone DNA polymerase, Escherichia coli pol V

The damage-inducible UmuD′ and UmuC proteins are required for most SOS mutagenesis in Escherichia coli. Our recent assay to reconstitute this process in vitro, using a native UmuD′(2)C complex, revealed that the highly purified preparation contained DNA polymerase activity. Here we eliminate the pos...

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Publikašuvnnas:Proc Natl Acad Sci U S A
Váldodahkkit: Tang, Mengjia, Shen, Xuan, Frank, Ekaterina G., O’Donnell, Mike, Woodgate, Roger, Goodman, Myron F.
Materiálatiipa: Artigo
Giella:Inglês
Almmustuhtton: National Academy of Sciences 1999
Fáttát:
Liŋkkat:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC17708/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10430871/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.16.8919
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