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Use of immunofluorescence to visualize cell-specific gene expression during sporulation in Bacillus subtilis.

We have adapted immunofluorescence microscopy for use in Bacillus subtilis and have employed this procedure for visualizing cell-specific gene expression at early to intermediate stages of sporulation. Sporangia were doubly stained with propidium iodide to visualize the forespore and mother cell nuc...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Harry, E J, Pogliano, K, Losick, R
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1995
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177040/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7768847/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.12.3386-3393.1995
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