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Use of multiple displacement amplification to amplify genomic DNA before sequencing of the α and β haemoglobin genes

Aims: To evaluate the technique of multiple displacement amplification (MDA) for whole genome amplification from small volume blood samples before sequencing in a clinical test to identify haemoglobin gene mutations. Methods: Phage φ29 DNA polymerase was used to perform MDA, starting with either 1 μ...

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Bibliografiske detaljer
Main Authors: Mai, M, Hoyer, J D, McClure, R F
Format: Artigo
Sprog:Inglês
Udgivet: Copyright 2004 Journal of Clinical Pathology 2004
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1770312/
https://ncbi.nlm.nih.gov/pubmed/15166271
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1136/jcp.2003.014704
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