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Use of multiple displacement amplification to amplify genomic DNA before sequencing of the α and β haemoglobin genes
Aims: To evaluate the technique of multiple displacement amplification (MDA) for whole genome amplification from small volume blood samples before sequencing in a clinical test to identify haemoglobin gene mutations. Methods: Phage φ29 DNA polymerase was used to perform MDA, starting with either 1 μ...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Copyright 2004 Journal of Clinical Pathology
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1770312/ https://ncbi.nlm.nih.gov/pubmed/15166271 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1136/jcp.2003.014704 |
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