Identification of Lrp-regulated genes by inverse PCR and sequencing: regulation of two mal operons of Escherichia coli by leucine-responsive regulatory protein.

We have used the technique of inverse PCR to identify Escherichia coli chromosomal genes carrying Lrp-regulated inserts. This technique revealed that malT, malEFG, and malB-lamB-malK are all activated two- to fivefold by Lrp and confirmed that Lrp regulates expression of the leuDBCA and livHJKG oper...

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Hauptverfasser: Tchetina, E, Newman, E B
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1995
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC176937/
https://ncbi.nlm.nih.gov/pubmed/7751276
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