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Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth.

NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mut...

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Vydáno v:J Bacteriol
Hlavní autoři: Ras, J, Van Ophem, P W, Reijnders, W N, Van Spanning, R J, Duine, J A, Stouthamer, A H, Harms, N
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 1995
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC176581/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7798140/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.1.247-251.1995
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