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A new screen for protein interactions reveals that the Saccharomyces cerevisiae high mobility group proteins Nhp6A/B are involved in the regulation of the GAL1 promoter

The split-ubiquitin assay detects protein interactions in vivo. To identify proteins interacting with Gal4p and Tup1p, two transcriptional regulators, we converted the split-ubiquitin assay into a generally applicable screen for binding partners of specific proteins in vivo. A library of genomic Sac...

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Bibliografische gegevens
Hoofdauteurs: Laser, Heike, Bongards, Christine, Schüller, Jutta, Heck, Stephanie, Johnsson, Nils, Lehming, Norbert
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: The National Academy of Sciences 2000
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC17644/
https://ncbi.nlm.nih.gov/pubmed/11095729
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