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An improved method to identify BAC clones using pooled overgos
Hybridization using overgo probes is an established approach for screening arrayed bacterial artificial chromosome (BAC) libraries. We have improved the use of overgos by increasing the yield of positive clones using reduced levels of radioisotopes and enzyme. The strategy involves labeling with all...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1761434/ https://ncbi.nlm.nih.gov/pubmed/17151072 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl920 |
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