Analysis of the Legionella pneumophila fliI gene: intracellular growth of a defined mutant defective for flagellum biosynthesis.

Using a PCR-based strategy and degenerate oligonucleotides, we isolated a Legionella pneumophila gene that showed high sequence similarity to members of the fliI gene family. An insertion mutation that disrupted the fliI open reading frame was recombined onto the L. pneumophila chromosome and analyz...

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Detalhes bibliográficos

Publicado no:	Infect Immun
Principais autores:	Merriam, J J, Mathur, R, Maxfield-Boumil, R, Isberg, R R
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC175352/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9169800/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.65.6.2497-2501.1997
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