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Cloning and sequence analysis of the pfl gene encoding pyruvate formate-lyase from Streptococcus mutans.

We have isolated a sorbitol-negative mutant of Streptococcus mutans GS-5 following random mutagenesis with plasmid pVA891 clone banks. This mutant did not metabolize sorbitol anaerobically but did so aerobically. A 10-kb chromosomal DNA fragment flanking the pVA891 insertion was deleted in this muta...

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Bibliographische Detailangaben
Hauptverfasser: Yamamoto, Y, Sato, Y, Takahashi-Abbe, S, Abbe, K, Yamada, T, Kizaki, H
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1996
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC173775/
https://ncbi.nlm.nih.gov/pubmed/8550181
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