Functional domains of Pseudomonas aeruginosa exoenzyme S.

Recombinant exoenzyme S (rHisExoS) of Pseudomonas aeruginosa was expressed in Escherichia coli as a soluble, cytosolic His fusion protein. rHisExoS was purified by Ni(2+)-affinity chromatography in the presence of protease inhibitors without detectable degradation. rHisExoS possessed a specific acti...

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Bibliographic Details
Main Authors: Knight, D A, Finck-Barbançon, V, Kulich, S M, Barbieri, J T
Format: Artigo
Language:Inglês
Published: 1995
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC173434/
https://ncbi.nlm.nih.gov/pubmed/7622246
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