Directed genomic integration in Actinobacillus actinomycetemcomitans: generation of defined leukotoxin-negative mutants.

To develop targeted gene integration in the periodontal pathogen Actinobacillus actinomycetemcomitans, a ColE1-based, spectinomycin-resistant plasmid containing a segment of the leukotoxin gene was electroporated into strain JP2. In all of the stable spectinomycin-resistant transformants that arose,...

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Main Authors: Kolodrubetz, D, Phillips, L H, Ezzo, P J, Kraig, E
Format: Artigo
Sprog:Inglês
Udgivet: 1995
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC173374/
https://ncbi.nlm.nih.gov/pubmed/7790100
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