Directed genomic integration in Actinobacillus actinomycetemcomitans: generation of defined leukotoxin-negative mutants.

To develop targeted gene integration in the periodontal pathogen Actinobacillus actinomycetemcomitans, a ColE1-based, spectinomycin-resistant plasmid containing a segment of the leukotoxin gene was electroporated into strain JP2. In all of the stable spectinomycin-resistant transformants that arose,...

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Detalhes bibliográficos

Publicado no:	Infect Immun
Principais autores:	Kolodrubetz, D, Phillips, L H, Ezzo, P J, Kraig, E
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC173374/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7790100/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.63.7.2780-2784.1995
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