Characterization of outer membranes isolated from Borrelia burgdorferi, the Lyme disease spirochete.

The lack of methods for isolating Borrelia burgdorferi outer membranes (OMs) has hindered efforts to characterize borrelial surface-exposed proteins. Here we isolated OMs by immersion of motile spirochetes in hypertonic sucrose followed by isopycnic ultracentrifugation of the plasmolyzed cells. The...

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Veröffentlicht in:	Infect Immun
Hauptverfasser:	Radolf, J D, Goldberg, M S, Bourell, K, Baker, S I, Jones, J D, Norgard, M V
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1995
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC173280/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7768594/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.63.6.2154-2163.1995
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