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Evaluation of extraction methods from paraffin wax embedded tissues for PCR amplification of human and viral DNA
Aim—To evaluate the efficiency of phenol/chloroform, microwave, and Qiagen spin column based DNA extractions from paraffin wax embedded tissue for use in the polymerase chain reaction (PCR). In addition, to assess the reliability of amplifying a housekeeping gene to indicate successful viral DNA ext...
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| Autori principali: | , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2001
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1731425/ https://ncbi.nlm.nih.gov/pubmed/11328843 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1136/jcp.54.5.401 |
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