Resolution of a missense mutant in human genomic DNA by denaturing gradient gel electrophoresis and direct sequencing using in vitro DNA amplification: HPRT Munich.

The combination of denaturing gradient gel electrophoresis (DGGE) and in vitro DNA amplification has allowed us to (1) localize a DNA mutation to a given 100-bp region of the human genome and (2) rapidly sequence the DNA without cloning. DGGE showed that a mutation had occurred, but the technique re...

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Detalhes bibliográficos
Main Authors: Cariello, N F, Scott, J K, Kat, A G, Thilly, W G, Keohavong, P
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1715170/
https://ncbi.nlm.nih.gov/pubmed/3358423
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