Semiquantitative Plate Assay for Determination of Cellulase Production by Trichoderma viride

A plate clearing assay was devised to screen for high-producing cellulase mutants of Trichoderma viride. The method employs (i) the use of either rose bengal or oxgall to limit colony size and (ii) Phosfon D (tributyl-2, 4-dichloroben-zylphosphonium chloride) to enhance cellulase detection, in combi...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Montenecourt, Bland S., Eveleigh, Douglas E.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1977
Schlagworte:	Metabolism and Products
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC170619/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16345184/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.33.1.178-183.1977
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