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Dilution assessment of cervicovaginal secretions collected by vaginal washing to evaluate mucosal shedding of free human immunodeficiency virus.

A 10 mM concentration of lithium does not interfere with reverse transcription (RT) or PCR. Sampling of cervicovaginal fluid by vaginal washing, with lithium (10 mM) in the washing buffer as a marker of dilution, may be utilized to accurately determine in HIV-infected women, by quantitative RT-PCR,...

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Udgivet i:Clin Diagn Lab Immunol
Principais autores: Mohamed, A S, Becquart, P, Hocini, H, Métais, P, Kazatchkine, M, Bélec, L
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1997
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC170612/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9302218/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/cdli.4.5.624-626.1997
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