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Precise quantitation of antinuclear antibodies on HEp-2 cells without the need for serial dilution.

Using HEp-2 cells as a substrate, we developed a method to quantitate antinuclear antibodies (ANA) by comparing the green fluorescence intensity of unknown samples with that of calibrated standards. Intensity was then converted to international units per milliliter by reference to a standard curve....

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Détails bibliographiques
Publié dans:Clin Diagn Lab Immunol
Auteurs principaux: Hollingsworth, P N, Dawkins, R I, Peter, J B
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1996
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC170353/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8807199/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/cdli.3.4.374-377.1996
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