Cloning of porcine cytokine-specific cDNAs and detection of porcine tumor necrosis factor alpha, interleukin 6 (IL-6), and IL-1 beta gene expression by reverse transcription PCR and chemiluminescence hybridization.

A reverse transcription PCR assay with porcine cytokine-specific primers was developed to clone cDNA fragments and generate cDNA probes that were specific for porcine tumor necrosis factor alpha (TNF-alpha), interleukin 6 (IL-6), and IL-1 beta. The specificities of the cDNA PCR products were confirm...

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Pubblicato in:	Clin Diagn Lab Immunol
Autori principali:	Vézina, S A, Roberge, D, Fournier, M, Dea, S, Oth, D, Archambault, D
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1995
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC170217/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8574826/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/cdli.2.6.665-671.1995
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