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A Real-Time PCR Method for Quantifying Viable Ascaris Eggs Using the First Internally Transcribed Spacer Region of Ribosomal DNA

Worldwide, 1.4 billion people are infected with the intestinal worm Ascaris lumbricoides. As a result, Ascaris eggs are commonly found in wastewater and sludges. The current microscopy method for detecting viable Ascaris eggs is time- and labor-intensive. The goal of this study was to develop a real...

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Autors principals: Pecson, Brian M., Barrios, José Antonio, Johnson, David R., Nelson, Kara L.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2006
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1694259/
https://ncbi.nlm.nih.gov/pubmed/17056687
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01983-06
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