In Vitro Deoxynucleotidylation of the Terminal Protein of Streptomyces Linear Chromosomes

Single-stranded gaps at the 3′ ends of Streptomyces linear replicons are patched by DNA synthesis primed by terminal proteins (TP) during replication. We devised an in vitro system that specifically incorporated dCMP, the first nucleotide at the 5′ ends, onto a threonine residue of the TP of Strepto...

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Bibliographic Details
Main Authors: Yang, Chien-Chin, Chen, Yi-Hong, Tsai, Hsiu-Hui, Huang, Chih-Hung, Huang, Tzu-Wen, Chen, Carton W.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2006
Subjects:
Genetics and Molecular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1694218/
https://ncbi.nlm.nih.gov/pubmed/17056688
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01857-06
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