Multiplex Real-Time PCR for Monitoring Heterobasidion annosum Colonization in Norway Spruce Clones That Differ in Disease Resistance

A multiplex real-time PCR assay was developed to monitor the dynamics of the Picea abies-Heterobasidion annosum pathosystem. Tissue cultures and 32-year-old trees with low or high resistance to this pathogen were used as the host material. Probes and primers were based on a laccase gene for the path...

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Detalhes bibliográficos
Main Authors: Hietala, Ari M., Eikenes, Morten, Kvaalen, Harald, Solheim, Halvor, Fossdal, Carl G.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC169156/
https://ncbi.nlm.nih.gov/pubmed/12902224
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.8.4413-4420.2003
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