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Construction of a Helicobacter pylori-Escherichia coli shuttle vector for gene transfer in Helicobacter pylori.

In this study, a Helicobacter pylori-Escherichia coli shuttle vector was constructed for transferring DNA into H. pylori. The smallest cryptic plasmid (1.2 kb), pHP489, among those harbored by 77 H. pylori isolates was selected as a base replicon for constructing vectors. HindIII-digested pHP489 was...

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Publicat a:Appl Environ Microbiol
Autors principals: Lee, W K, An, Y S, Kim, K H, Kim, S H, Song, J Y, Ryu, B D, Choi, Y J, Yoon, Y H, Baik, S C, Rhee, K H, Cho, M J
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 1997
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168813/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9406406/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.12.4866-4871.1997
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