Cloning, sequencing, and expression of the gene encoding extracellular alpha-amylase from Pyrococcus furiosus and biochemical characterization of the recombinant enzyme.

The gene encoding the hyperthermophilic extracellular alpha-amylase from Pyrococcus furiosus was cloned by activity screening in Escherichia coli. The gene encoded a single 460-residue polypeptide chain. The polypeptide contained a 26-residue signal peptide, indicating that this Pyrococcus alpha-amy...

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Detalles Bibliográficos

Publicado en:	Appl Environ Microbiol
Autores principales:	Dong, G, Vieille, C, Savchenko, A, Zeikus, J G
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 1997
Materias:	Research Article
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168662/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9293008/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.9.3569-3576.1997
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