Molecular analysis of the Rhodococcus sp. strain H1 her gene and characterization of its product, a heroin esterase, expressed in Escherichia coli.

The structural gene for heroin esterase was cloned from Rhodococcus sp. strain H1 and expressed in Escherichia coli BL21(DE3). The purified enzyme was found to be a tetramer with an M(r) of 137,000 and an apparent K(m) of 0.88 mM for 6-acetylmorphine. The G-x-S-x-G motif was observed in the deduced...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Rathbone, D A, Holt, P J, Lowe, C R, Bruce, N C
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1997
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168495/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9143135/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.5.2062-2066.1997
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