PCR assay based on a microsatellite-containing locus for detection and quantification of Epichloë endophytes in grass tissue.

A PCR assay which allows detection and quantification of Epichloë endophytes in tissues of the grass Bromus erectus is described. PCR with specific primers flanking a microsatellite-containing locus (MS primers) amplified fragments 300 to 400 bp in length from as little as 1.0 pg of fungal genomic D...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Groppe, K, Boller, T
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1997
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168446/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9097449/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.4.1543-1550.1997
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