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Detection system for Escherichia coli-specific virulence genes: absence of virulence determinants in B and C strains.

We describe a rational approach to simultaneously test Escherichia coli strains for the presence of known virulence genes in a reverse dot blot procedure. Specific segments of virulence genes of E. coli designed to have similar hybridization parameters were subcloned on plasmids and subsequently amp...

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Published in:Appl Environ Microbiol
Main Authors: Kuhnert, P, Hacker, J, Mühldorfer, I, Burnens, A P, Nicolet, J, Frey, J
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1997
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Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168360/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9023948/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.2.703-709.1997
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