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Direct detection of dystrophin gene rearrangements by analysis of dystrophin mRNA in peripheral blood lymphocytes.

Using 10 overlapping nested sets of primers and using peripheral blood lymphocyte (PBL) total RNA as template, we have developed a system, based on PCR, which allows the rapid production of double-stranded cDNA corresponding to the entire coding sequence of the dystrophin gene. The product can be vi...

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Bibliografiske detaljer
Main Authors: Roberts, R G, Barby, T F, Manners, E, Bobrow, M, Bentley, D R
Format: Artigo
Sprog:Inglês
Udgivet: 1991
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1683309/
https://ncbi.nlm.nih.gov/pubmed/1867192
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