Detection of viable Giardia cysts by amplification of heat shock-induced mRNA.

Primers obtained from gene sequences coding for heat shock proteins (HSP) were used to specifically detect enteric protozoans of the genus Giardia. The HSP primers amplified Giardia DNA or the corresponding RNA sequences obtained from lysed cysts and gave a 163-bp product. Since the presence of the...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Abbaszadegan, M, Huber, M S, Gerba, C P, Pepper, I L
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1997
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168324/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8979360/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.1.324-328.1997
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