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Cloning of genes encoding alpha-L-arabinofuranosidase and beta-xylosidase from Trichoderma reesei by expression in Saccharomyces cerevisiae.

A cDNA expression library of Trichoderma reesei RutC-30 was constructed in the yeast Saccharomyces cerevisiae. Two genes, abf1 and bxl1, were isolated by screening the yeast library for extracellular alpha-L-arabinofuranosidase activity with the substrate p-nitrophenyl-alpha-L-arabinofuranoside. The...

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Autores principales: Margolles-Clark, E, Tenkanen, M, Nakari-Setälä, T, Penttilä, M
Formato: Artigo
Lenguaje:Inglês
Publicado: 1996
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC168192/
https://ncbi.nlm.nih.gov/pubmed/8837440
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