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A simple procedure for isolation of cloning vectors and endogenous plasmids from viridans group streptococci and Staphylococcus aureus.

Isolation of plasmid DNA from viridans group streptococci is difficult, and preparations are often heavily contaminated with chromosomal DNA. We developed a simple protocol to isolate pure plasmid DNA for use in different molecular techniques, including automated sequencing. The protocol is also app...

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Bibliographic Details
Main Authors: Vriesema, A J, Zaat, S A, Dankert, J
Format: Artigo
Language:Inglês
Published: 1996
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC168154/
https://ncbi.nlm.nih.gov/pubmed/8795248
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