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A simple procedure for isolation of cloning vectors and endogenous plasmids from viridans group streptococci and Staphylococcus aureus.

Isolation of plasmid DNA from viridans group streptococci is difficult, and preparations are often heavily contaminated with chromosomal DNA. We developed a simple protocol to isolate pure plasmid DNA for use in different molecular techniques, including automated sequencing. The protocol is also app...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Vriesema, A J, Zaat, S A, Dankert, J
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1996
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC168154/
https://ncbi.nlm.nih.gov/pubmed/8795248
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