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Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection
We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosom...
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| Main Authors: | , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1681482/ https://ncbi.nlm.nih.gov/pubmed/16738554 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/msb4100050 |
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